How the PRISMA 2020 checklist is organised
The PRISMA 2020 checklist maps onto the standard sections of a research manuscript, so working through it in order mirrors the way you write the paper. The 27 items run from the title and abstract, through the introduction and a detailed methods section, into the results, the discussion, and a final other information block covering registration, funding, competing interests, and data availability.
The heaviest section is methods, items 5 to 15, because PRISMA 2020 expects you to report your eligibility criteria, every information source, the full search strategy, the selection and data collection processes, risk of bias methods, and how you synthesised and graded the evidence. Getting these right is what lets a reader judge whether your review is reproducible. For a full explanation of each requirement, our item-by-item guide to the PRISMA 2020 checklist walks through what every line asks for.
Where the flow diagram fits in
Item 16, study selection, sits in the results section and asks you to describe the outcome of your search and screening. Reviewers expect this as a PRISMA 2020 flow diagram showing records identified, duplicates removed, records screened and excluded, reports assessed for eligibility, and studies included. The checklist tells you the diagram is required; the diagram itself is a separate figure you build alongside the manuscript. You can produce it with the free flow diagram generator on this site and attach it against item 16.
How to use this checklist before submission
- Mark each item as reported once it is written. Tick an item only when the manuscript actually contains it, not when you intend to add it.
- Use not applicable sparingly and explain it. Some items genuinely will not apply, such as certainty assessment if you did not grade the evidence. State that in the text rather than leaving the item silent.
- Record the page or section number. Journals ask for the location of each item on the submitted checklist, so keep a note of where each one lives as you go.
- Reconcile item 16 with your flow diagram. The numbers in the diagram must match the selection narrative in your results.